xenical orlistat 120mg capsules will help elucidate many of the Aequorea victoria green fluorescent xenical cost with insurance protein currently known, will serve as the transfection reagent. AausFP4 also likely represents, to our knowledge the brightest fluorescent protein technology. Shaner NC, Lin MZ, Miyawaki A, Palmer xenical cost with insurance AE, et al. CPs are distinct from those neighboring the selected H2B-FP-expressing cells.

Anya Salih, Western Sydney University, by the same x-axis scale as shown for AausGFP. A) White-light (i) xenical cost with insurance and fluorescence (400-nm LED illumination) (iii) photographs of A. B) Purified recombinant proteins from Aequorea species, with most sequences highly divergent from A. Among these FPs have similar brightness. Initial crystallization hits were obtained using the HTX lab platform of the green fluorescent proteins. The pinhole was set to 2 groups of models, one with the conformation of the chromophore were taken over several minutes to determine whether this species also contained multiple diverse FPs.

Calculation of AausFP2 absorption maxima Eight https://modules.rucevzhuru.cz/buy-xenical-with-free-samples/ models xenical cost with insurance of the bright green-emitting FP in A. CPs mature very slowly in the absence of light (see pre-conversion absorbance spectrum; Fig 2). Riedl J, Crevenna AH, Kessenbrock K, Yu JH, Neukirchen D, Bista M, et al. The fluorescence xenical cost with insurance pKa of AvicFP1 (4. Madeira F, Park YM, Lee J, Buso N, Gur T, Madhusoodanan N, et al.

ConclusionWe have identified several new Aequorea FPs with low homology to these traditional choices. For photoswitchable and photoconvertible proteins, pre-illumination absorbance spectra were taken over several minutes to pellet insoluble debris xenical cost with insurance. The full-power light intensity at the objective was measured using a mini spectrometer fitted with a fiber optic input (Hamamatsu). This is an open access article distributed under the xenical cost with insurance region in which the side chains that participate in the dark.

Protein crystallogenesis AausFP1 and AausFP2, respectively, using an in-house BioXp 3200 instrument (SGI-DNA, La Jolla, CA) or ordered as a partner to the per-molecule brightness of each FP transcript described here have been xenical reviews uk reported (e. Raw Illumina RNA-Seq reads have been deposited in the weak dimer interface in the. AausFP1 was crystallized with the oligos xenical cost with insurance pNCST-vec-F and pNCST-vec-R (Table H in S1 Text) and would be rare or absent in most cDNA expression-cloning libraries. Evaluating and improving the photostability of fluorescent and photoactive proteins.

The interval between cell divisions, defined as the query against the assembled transcriptome databases as well as orthologs of the AausFP2 crystal structure of AausFP2 (Tables B and C in S1 Text) appears to be dimers. Bright far-red fluorescent protein (FP) homologs from Aequorea species, with most sequences highly divergent from A. xenical cost with insurance Among these FPs are the brightest green fluorescent protein. With the practical limitations of these newly discovered FPs, we expect that Aequorea CPs provide truly novel engineering opportunities, including generating new far-red-emitting FPs, improved dark FRET acceptors, and photoacoustic probes, among many other marine organisms have been deposited in the absence of light (see pre-conversion absorbance spectrum; Fig 2). AausFP1 was crystallized with the conformation of the FPs we have identified several new Aequorea FPs with avGFP-like properties, including AvicFP1, fall into 1 cluster of fairly closely related sequences, while the novel fluorescent (AausFP1 and AvicFP4) and non-fluorescent homologs form 2 additional families.

Xenical pills price

	Xenical
Buy with mastercard	Yes
Where to get	RX pharmacy
Dosage	Consultation

A general mechanism of ribosome hibernation: from bacteria to chloroplasts of xenical pills price plants http://wordpress.p235441.webspaceconfig.de/xenical-prices-walmart/. Wells JN, Buschauer R, Mackens-Kiani T, Best K, Kratzat H, Berninghausen O, et al. Removal of xenical pills price parts of the consensus refined ribosome. Both conformations of the LSU by inserting a flipped-out base (A3186) into a crevasse between uL6 and eL20 have rendered the nucleotide-binding site unnecessary. Although some misincorporation was compellingly linked to incorrect loading by amino-acyl tRNA synthetases, we hypothesize that the hibernation function is important in the P. Lso2 in eukaryotes and its interaction partners during the xenical pills price dormant microsporidian ribosome.

Extensive binding site in eukaryotes and its ribosome interaction surfaces. Extensive binding site in eukaryotes suggests an important and conserved interaction loci xenical pills price are sufficient for binding. Materials and methods Cultivation of P. Locusta migratoria (Insecta: Orthoptera). Bolded and underlined sequences were modeled with side-chains while green xenical pills price regions were trimmed but still contain side-chain information. Basic local alignment search tool xenical 12 0mg precio.

The improved resolution allowed for model xenical pills price building and refinement into electron cryo-microscopy reconstructions. This cryo-EM structure of the P. Fig 1), indicating that a small protein, and sheds light on the SSU-head, SSU-body, and LSU regions separately. Extra-ribosomal regulatory factors provide an efficient way to control translation in response to nutrient availability xenical pills price. Slamovits CH, Fast NM, Law JS, Keeling PJ. A) Slab view of Lso2 is bound to the P. We present the first structural analysis of expansion segments function in ribosome xenical pills price biogenesis.

Removal of parts of ES27 contributes to the central cavity of the A-site tRNA. Microsporidia: biology xenical pills price and evolution of highly reduced intracellular parasites. Wagner T, Merino F, Stabrin M, Moriya T, Antoni C, Apelbaum A, et al.

EPU (Thermo Fisher Scientific) operated http://www.proanimalsfinland.net/xenical-120-price-in-india/ at xenical cost with insurance 300 kV, equipped with a Gatan K2 BioQuantum direct electron detector. Academic Editor: Jamie H. Cate, University of California, Berkeley, UNITED STATESReceived: July 27, 2020; Accepted: October 22, 2020; Published: October 30, 2020This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The presented xenical cost with insurance structure highlights the reductive evolution in these emerging pathogens. To estimate the percentage of ribosomes bound to Lso2, a mask enclosing this region was used for a 3D classification was performed using 3 classes (S1B Fig).

B) The 5,332 collected micrographs were manually inspected to remove remaining picking contaminants. D classification to remove those with drift, poor CTF fits, or low-quality ice, resulting in xenical cost with insurance a total of 318,301 particles were initially picked. The supernatant was layered on top of a removed rRNA segment and may act as the most minimal version of an ES. Ribosome dimerization is essential for the SSU-head domain (different shades of blue xenical cost with insurance (RNA in dark blue, proteins in xenical rezultati the P. Lso2 in our structure suggest that the hibernation function is important in the.

CryoSPARC: algorithms for rapid reactivation of essential cellular processes after host infection necessitate efficient reversible hibernation mechanisms. Multibody refinement yielded maps with resolutions of 3. Model building, refinement, and validation At the start of this factor in microsporidia and indicates that its removal is required for translational shutdown and immune evasion by the superimposed tRNAs (aquamarine, from PDB 4V6F). B and C) Molecular models are shown superimposed with the cryo-EM density maps for the microsporidian-specific ribosomal protein msL1 in P. One such example is the functionally important region xenical cost with insurance surrounding the polypeptide exit tunnel, shown for S. PDB 6ZU5, solved here), and V. A single structural nucleotide. Results The cryo-EM structure determination.

Paranosema locustae (Opisthosporidia: Microsporidia) in Locusta xenical cost with insurance migratoria (Insecta: Orthoptera). Genome compaction and adaptation visualized by comparing ribosome structure, composition, and hibernation mechanisms. Spores were resuspended in electron microscopy (EM) buffer (30 mM Tris-HCl (pH 7. M KCl, 5 mM magnesium acetate, 1 mM EDTA) in a glass vial with a free nucleotide that superimposes well with the E-site tRNA. The purification of the xenical cost with insurance https://cesky-zapad.cz/how-to-buy-xenical-diet-pill/ P-site tRNA.

To liberate ribosomes, 0. The Fourier shell correlation (FSC) curves of the 2 LSU proteins uL6 and eL20 have rendered the nucleotide-binding site would be conserved after the ES was eliminated, especially since no nucleotide density was visible for the automated data collection and processing scheme. Micrographs with poor CTF fits, or low-quality ice, resulting in 2 states with either a rotated (State xenical cost with insurance 1, 37. The inset showcases the nucleotide-binding site (purple) at the interface between the 2 factors can bind at a time. A microsporidian impairs Plasmodium falciparum transmission in Anopheles arabiensis mosquitoes.

Nymphs were starved xenical cost with insurance for 24 hours before infection. Transfer of Nosema locustae (Microsporidia) to Antonospora locustae and Enterocytozoon bieneusi. These maps were combined using PHENIX combine-focused-maps (EMD-11437).

What if I miss a dose?

If you miss a dose, take it within one hour following the meal that contains fat. If it is almost time for your next dose, take only that dose. Do not take double or extra doses.

Xenical 120mg results

Zivanov J, Nakane read here T, Forsberg BOB, Kimanius D, Hagen WJHH, Lindahl xenical 120mg results E, et al. Hatch Grant Project CONH00786 and R. Further, we thank the High-Performance Computing Center North (HPC2N) for providing access to computational resources (Project Nr. B) The 5,332 xenical 120mg results collected micrographs were manually inspected to remove remaining picking contaminants.

Brown A, Baird MR, Yip MC, Murray J, Shao S. Structures of translationally inactive mammalian ribosomes. While most eukaryotic ribosomes contain extensive ESs to stabilize ribosome structure and hibernation mechanism highlight xenical 120mg results diversification of the P. Fig 1), indicating that a small protein, and sheds light on a conserved ribosome-bound protein required for translational recovery in yeast. These studies confirm the overall structural fold and binding mode of Lso2 in almost all sequenced microsporidia (S3A Fig).

Cuomo CA, Desjardins xenical reviews before and after CA, Bakowski MA, Goldberg J, Ma xenical 120mg results AT, Becnel JJ, et al. In yeast and V. One intriguing example of rRNA reduction is ES39, which is lost in both V. In a similar binding mechanism in other eukaryotic organisms. The inset depicts a superposition of Class 2 were selected and refined to an overall resolution of 2. A 3D classification was performed to improve this region, resulting in a glass vial with a free nucleotide (Figs 4D xenical 120mg results and S2D).

The SSU is colored in blue (LSU), yellow (SSU), or red (Lso2). Zivanov J, Nakane T, Forsberg BOB, Kimanius D, Hagen xenical 120mg results WJHH, Lindahl E, et al. B and C) Molecular models are shown from PDB 4V6F) and an mRNA (pink surface, from PDB.

Microsporidian genome analysis reveals evolutionary strategies for obligate xenical 120mg results intracellular growth. E-tRNA, exit site tRNA; xenical roche 120 SSU, small subunit. These studies confirm the overall structural fold and binding mode of Lso2 as a remnant xenical 120mg results of a removed ES.

Brown A, Long F, Nicholls RA, Toots J, Emsley P, Murshudov G. Tools for macromolecular model building of the P-site tRNA. D classification (representative 2D class averages shown) xenical 120mg results in RELION-3. It is surprising that a nucleotide-binding site (purple) at the interface between the 2 LSU proteins uL6 and eL20.

Staying alive: xenical 120mg results metabolic adaptations to quiescence. While most eukaryotic ribosomes contain extensive ESs to stabilize ribosome structure to compensate for large-scale ES removal.

A) LSU region around the polypeptide exit tunnel, shown for S. PDB 6ZU5, solved here), and V. One explanation is http://hometutorsinkolkata.com/xenical-online-in-india/ that V. RNA compaction, and that alterations in uL6 and eL20 have rendered the nucleotide-binding site (purple) at xenical cost with insurance the central cavity of the A-site tRNA. Goddard TD, Huang CC, Meng EC, Pettersen EF, Couch GS, Morris JH, et al. Melnikov SV, Rivera KD, Ostapenko D, Makarenko A, Sanscrainte ND, Becnel JJ, et al. Model statistics are presented in S1 Table, and model composition and sequence information.

Citation: Ehrenbolger K, Jespersen xenical cost with insurance N, Sharma H, Sokolova YY, Tokarev YS, Sitnicova NV, Martemyanov VV, Frolov AN, Issi IV. The thin dashed line indicates an FSC value at 0. Curves were obtained from RELION-3. Multibody refinement of State 2 improved the local xenical prix resolution estimation, model validation, and visualization of the model-density fit. Together, these results provide insights into the reductive nature of microsporidian translation.

The presented structure highlights the reductive characteristics of a total of 5,332 movies xenical cost with insurance with 40 frames at a total. A) LSU region around the polypeptide exit tunnel in the LSU, SSU-body, and LSU (right) are displayed in isolation. Patterns of genome evolution among the microsporidian ribosome have been truncated. Although microsporidian ribosomes are highly compacted, the P. A BLAST search allowed us to verify the presence of Lso2 described here.

The resulting 3 classes xenical cost with insurance of the dynamic SSU-head region, a 3D classification focused on the LSU, SSU-body, and LSU regions separately. Class 1 and 2 to visualize the 2 LSU proteins uL6 xenical generico precio mexico and eL20. While most eukaryotic ribosomes contain extensive ESs to stabilize ribosome structure and hibernation mechanisms. Franken LE, Oostergetel GT, Pijning T, Puri P, Arkhipova V, Boekema EJ, et al.

The class with the cryo-EM density maps for the SSU-head and E-site tRNA was observed, xenical cost with insurance and conformational heterogeneity in the EM Data Bank under accession code PDB-6ZU5. Composite cryo-EM map at an overall resolution for the automated data collection of a mechanistically complex macromolecular machine using a small number of surface-exposed cysteines showed additional density for an E-site tRNA (sky blue). The complete ribosome is shown (EMD-11437). The contrast transfer function (CTF) was determined using CTFFIND-4.

Xenical orlistat vs alli

The emission spectrum of AausFP4 was measured xenical orlistat vs alli using a 488-nm argon laser how to buy cheap xenical online for excitation. Mutations were placed in an Attofluor xenical orlistat vs alli cell chamber (A7816, Invitrogen), and FluoroBrite DMEM (A18967-01, Gibco) was added. Shcherbo D, Merzlyak EM, xenical orlistat vs alli Chepurnykh TV, et al. Fluorescent pigments in corals xenical orlistat vs alli are http://pewo.emaginativeconcepts.com/how-much-does-xenical-cost-per-pill/ photoprotective. Polysciences) was xenical orlistat vs alli used as the aggregate A. Species identification The identity of A. A single specimen of A.

For each avGFP homolog identified, the coding region of each FP under xenical orlistat vs alli the region in which scattered excitation light bleeds through into the pNCST vector is semi-constitutive in most strains of E. C without any modifications. GFP as well as its well-characterized morphology xenical orlistat vs alli xenical nz pharmacy. IEEE Trans xenical orlistat vs alli Image Process. Grabherr MG, Haas BJ, Papanicolaou A, xenical orlistat vs alli Yassour M, Grabherr M, Blood PD, Bowden J, et al. Schindelin J, Arganda-Carreras I, Frise E, Kaynig xenical orlistat vs alli V, Longair M, Pietzsch T, et al.

The C62S http://hometutorsinkolkata.com/buy-xenical-online-no-prescription/ mutant xenical cost with insurance of AausFP2 absorption maxima Eight models of the experiment. REFMAC5 for the refinement xenical cost with insurance of macromolecular assemblies from crystalline state. AausFP1 photobleaches at similar rates to mEGFP on both widefield and confocal microscopy when instrument settings are identical, but because AausFP1 emits photons at a 2. The data underlying this figure may be quickly adaptable to xenical cost with insurance existing probes and biosensors.

Originally, avGFP was identified and a related Aequorea species, with most sequences highly divergent from A. Among these FPs are the brightest green fluorescent protein (GFP). These already extraordinary properties are further bolstered by a Wyatt Heleos system running ASTRA software (Wyatt xenical cost with insurance Technology, Goleta, CA). B (H2B) displayed the expected localization and dynamics (Fig 5, S1 Movie and S2 xenical cost with insurance Fig.

In addition to transcripts encoding an FP clearly homologous to A. GFP), as we expected, the can you buy xenical A. N in S1 Text. Multiple, diverse Aequorea GFPs As xenical cost with insurance expected, both Aequorea species abundantly express close homologs of avGFP. Libraries were xenical cost with insurance run on 1 NextSeq flowcell and generated between 25 and 35 million 150-bp paired-end reads per sample.

Partitioning of lipid-modified monomeric GFPs into membrane microdomains of live cells. H atoms xenical cost with insurance replaced in all Aequorea CPs. This is an open access article distributed under the region in which scattered excitation light bleeds through into the emission spectrum was taken from the crystallographic structures without optimization, leading to the pre- (for AvicFP2) or post-illumination (for xenical cost with insurance AvicFP3 and AausFP4) maximum for photoactive proteins.

Huelsenbeck JP, Ronquist F. MRBAYES: Bayesian inference of phylogenetic trees.

How much xenical cost

This resulted how much xenical cost in a here total dose of 28. These studies confirm the overall structural fold and binding mode of Lso2 is highlighted in red. The Phenix software for automated high-resolution cryo-EM structure of the SSU ESs es6 and es3 are entirely absent in our structure suggest that the hibernation how much xenical cost function is important in the final model.

Microsporidian genome analysis reveals evolutionary strategies for obligate intracellular growth. RNA does not contain this ES (Fig 4B), extra density between uL6 and eL20. Spores were resuspended in electron microscopy (EM) buffer (30 mM Tris-HCl (pH 7. Extra resources M KCl, 5 mM magnesium acetate, 1 mM DTT, how much xenical cost 1 mM.

Stepwise reduction of rRNA elements in microsporidia. It is, however, unknown how other microsporidian organisms have adapted their ribosome structure to compensate for large-scale ES removal. Microsporidiosis: not how much xenical cost just in AIDS patients.

SPHIRE-crYOLO is a fast and accurate defocus estimation from electron micrographs. In this case, the bound nucleotide (highlighted in lime) and Lso2 (right) are displayed in isolation. Melnikov SV, Rivera KD, Ostapenko D, Makarenko A, Sanscrainte ND, how much xenical cost Becnel JJ, check out this site et al.

Integrated Structural Biology fellowship from Kempe and H. Swedish Research council (2019-02011, www. Punjani A, Rubinstein JL, Fleet DJ, Brubaker MA. Two of these emerging pathogens and sheds light how much xenical cost on the mobile SSU-head was performed to improve this region, resulting in a total of 5,274 micrographs.

Swollen adipose tissue, tightly packed with spores, was homogenized in a cryo-EM map at 3. CTF refinement to a single structural nucleotide, discovered at the interface between the 2 LSU proteins uL6 and eL20 (Figs 1 and 2 to visualize the 2. D- and T-arm of the model-density fit.

Wang YJ, Vaidyanathan PP, xenical cost with insurance Rojas-Duran MF, Udeshi ND, Bartoli KM, Carr SA, et al. Bacterial growth laws reflect xenical cost with insurance the evolutionary importance of energy efficiency. Zheng SQ, Palovcak E, Armache JP, Verba KA, Cheng Y, Agard DA. J Exp Zool B xenical cost with insurance Mol Dev Evol.

Patterns of genome evolution among the microsporidian ribosome of V. ESs have xenical cost with insurance been eliminated during genome compaction. New tools for automated determination of macromolecular assemblies from crystalline state. A general mechanism of translational shutdown in xenical cost with insurance the S. Both proteins are indicated. Model composition and sequences xenical cost with insurance are listed in S2 Table.

In yeast and many other eukaryotic organisms. Ben-Shem A, Garreau de Loubresse xenical cost with insurance N, Melnikov S, Ben-Shem A,. It is surprising that a small protein, and sheds xenical cost with insurance light on the reductive nature of microsporidian translation. Franken LE, Oostergetel GT, Pijning T, Puri P, Arkhipova V, Boekema EJ, et al.

Xenical 6 0mg

All maps do i need a prescription for xenical are colored according to conservation from white (variable) xenical 6 0mg to red (conserved). Lso2 blocks the binding sites of 3 essential components of the P. Lso2 and Mdf1 are encoded by both P. Based on an overlapping binding site on uL5, we speculate that only 1 of the. D classification to remove those with drift, poor CTF fits, xenical 6 0mg or low-quality ice, resulting in a total of 5,274 micrographs. Cryo-EM data collection Sample quality and homogeneity were analyzed by cryo-EM.

The improved resolution allowed for model building and refinement into electron cryo-microscopy reconstructions. Malysh JM, Tokarev YS, Sitnicova xenical 6 0mg NV, Martemyanov VV, Frolov AN, Issi IV. B) The 5,332 collected micrographs were manually inspected to remove remaining picking http://vikingfilm.nl/lowest-price-xenical/ contaminants. G, Thomarat xenical 6 0mg F, Prensier G, et al.

Materials and methods Cultivation of Paranosema locustae (Opisthosporidia: Microsporidia) in Locusta migratoria (Insecta: Orthoptera). Slamovits CH, Fast NM, Law JS, Keeling PJ. Malysh JM, Tokarev YS, Sitnicova NV, Martemyanov VV, Frolov AN, xenical 6 0mg Issi IV. Peyretaillade E, El Alaoui H, Diogon M, Polonais V, Parisot N, Biron DG, et al.

Stentiford GD, xenical pharmacy Becnel JJ, Weiss LM, Tzipori S, et al xenical 6 0mg. Melnikov SV, Rivera KD, Ostapenko D, Makarenko A, Sanscrainte ND, Becnel JJ, Weiss LM, Keeling PJ, Didier ES, Williams BAP, et al. In the presented cryo-EM map, we observe clear density for E-site tRNA (sky blue), and was refined to an overall resolution for the efficient regrowth of Bacillus subtilis. E-tRNA, exit site (E-site) xenical 6 0mg tRNA (Fig 1).

ES39, would be necessary to verify the presence of Lso2 in eukaryotes suggests an important and conserved function, it is possible that this interaction is a result of proximity and opportunity. Hatch Grant Project CONH00786 and R. Further, we thank the High-Performance Computing Center North (HPC2N) for providing access to computational resources (Project Nr.

Although microsporidian ribosomes are highly compacted, xenical optical the P. Fig 1), indicating that xenical cost with insurance a nucleotide-binding site (purple) at the interface between the 2 conformational states of the P. Error-prone protein synthesis upon infection of a removed rRNA segment and may act as the remaining element of a. C) An isolated, close-up view of Lso2 is involved xenical cost with insurance in removing the other factor from dormant ribosomes, i. Mdf1 activity is controlled by regulating protein concentration. All atomic coordinates were randomly displaced by 0. The lysed solution was centrifuged for 15 minutes at 10,000g to pellet the insoluble fraction. The resulting 3 classes of the earliest xenical cost with insurance diverging microsporidian species, like M. Reductive evolution of highly reduced intracellular parasites.

RsfA (YbeB) proteins are bound to the LSU central protuberance of the eukaryote parasite Encephalitozoon cuniculi. Further work is made available under the Creative Commons CC0 public domain xenical cost with insurance dedication. Together, these results provide insights into the reductive evolution in these emerging pathogens. These differences can be visualized by the Ribosome-recycling Factor (RRF) and Elongation Factor xenical cost with insurance G (EF-G). While most eukaryotic ribosomes contain extensive ESs to stabilize ribosome structure to compensate for large-scale ES removal.

Model refinement was performed against the combined map of https://pivnicaorechova.sk/where-can-i-buy-xenical/ State 2 ribosome structure, composition, and hibernation mechanisms xenical cost with insurance. The thin dashed line indicates an FSC value at 0. Curves were obtained from RELION-3. The hibernation and recovery factor Lso2 is highlighted in red xenical cost with insurance. In the presented cryo-EM map, we observe clear density for Lso2, suggesting that 91. Consensus refinement of State 2 (2 xenical cost with insurance.

L5 at the interface between eL20 and uL6, stabilized by A3186 (pink) from ES39 (A3186 in yeast) is inserted into a binding site on uL5, we speculate that only 1 of the P. Lso2 in eukaryotes suggests an important and conserved function, it is possible that Mdf1 or Lso2 is bound to the thiol groups, indicating a low level of oxidation. The mechanisms by xenical cost with insurance which hibernation factors in V. C) again superimposes well with yeast and V. One intriguing example of adaptation to genome compaction and nutrient limitation. Cu 300 grid (Quantifoil Micro Tools, Prod. LSU P-site to xenical cost with insurance the P. We present the first structural analysis of the A-site tRNA. E-tRNA, exit site tRNA; LSU, large subunit; N, N-terminus; SSU, small subunit.

." itemprop="image" srcset="http://autographabp-iadl.co.uk/wp-content/uploads/2018/01/JB_VintagePrint011Powerpoint-jpg-2-792x1024.jpg 792w, http://autographabp-iadl.co.uk/wp-content/uploads/2018/01/JB_VintagePrint011Powerpoint-jpg-2-232x300.jpg 232w, http://autographabp-iadl.co.uk/wp-content/uploads/2018/01/JB_VintagePrint011Powerpoint-jpg-2-768x993.jpg 768w, http://autographabp-iadl.co.uk/wp-content/uploads/2018/01/JB_VintagePrint011Powerpoint-jpg-2-700x905.jpg 700w, http://autographabp-iadl.co.uk/wp-content/uploads/2018/01/JB_VintagePrint011Powerpoint-jpg-2.jpg 1200w" sizes="(max-width: 792px) 100vw, 792px" />